Studies of neural plasticity in motor disorders and the neural circuits underlying touch and pain.
Studies in the Seal laboratory are focused on delineating the neural circuitry that drives behavior normally and in disease. We aim to discover fundamental principals and mechanisms of the nervous system as well as identify novel treatment strategies for nervous system disorders. Our laboratory is currently studying: 1) peripheral and central pain circuits, 2) motor circuits affected by Parkinson’s disease and 3) peripheral and central auditory circuits
Experimental Approach: our laboratory uses mice as a model system and techniques such as virally-mediated DREADDs activation or inhibition of neurons, virally-mediated anterograde and retrograde circuit tracing, slice electrophysiology with optogenetics, confocal and 2-photon microscopy, molecular biology, histology, biochemistry and behavior.
For more details visit the Seal Lab website at seallab.neurobio.pitt.edu.
Neural circuits for pain: Recent advances and current views.
Peirs C, Seal RP.
Science. 2016 Nov 4;354(6312):578-584.
Illuminating the Gap: Neuronal Cross-Talk within Sensory Ganglia and Persistent Pain.
Neuron. 2016 Sep 7;91(5):950-1.
Do the distinct synaptic properties of VGLUTs shape pain?
Neurochem Int. 2016 Sep;98:82-8.
Loss of VGLUT3 Produces Circadian-Dependent Hyperdopaminergia and Ameliorates Motor Dysfunction and l-Dopa-Mediated Dyskinesias in a Model of Parkinson's Disease.
Divito CB, Steece-Collier K, Case DT, Williams SP, Stancati JA, Zhi L, Rubio ME, Sortwell CE, Collier TJ, Sulzer D, Edwards RH, Zhang H, Seal RP.
J.Neurosci. 2015 Nov 11; 35(45):14983-99.
Targeting Toll-like receptors to treat chronic pain.
Peirs C, Seal RP.
Nat Med. 2015 Nov 5;21(11):1251-2.
Dorsal Horn Circuits for Persistent Mechanical Pain.
Peirs C, Williams SPG, Zhao X, Walsh CE, Gedeon JY, Cagle NE, Goldring AC, Hioki H, Liu Z, Marell PS, Seal RP.
Neuron. 2015 Aug 19;87(4):797-812.
Striatal Cholinergic Neurotransmission Requires VGLUT3.
Nelson AB, Bussert TG, Kreitzer AC, Seal RP.
Neurosci. 2014 Jun 25;34(26):8772-7.
Striatal cholinergic interneurons drive GABA release from dopamine terminals.
Nelson AB, Hammack N, Yang CF, Shah NM, Seal RP, Kreitzer AC.
Neuron. 2014 Apr 2;82(1):63-70.
Restoration of hearing in the VGLUT3 knockout mouse using virally mediated gene therapy.
Akil O, Seal RP, Burke K, Wang C, Alem A, During MJ, Edwards RH, Lustig LR.
Neuron. 2012 Jul 26;75(2):283-93.
Injury-induced mechanical hypersensitivity requires C-low threshold mechanoreceptors.
Seal RP, Wang X, Guan Y, Raja SN, Woodbury CJ, Basbaum AI, Edwards RH.
Nature. 2009 Dec 3;462(7273):651-5.
Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3.
Seal RP, Akil O, Yi E, Weber CM, Grant L, Yoo J, Clause A, Kandler K, Noebels JL, Glowatzki E, Lustig LR, Edwards RH.
Neuron. 2008 Jan 24;57(2):263-75. 97-812.