Ion transporters in neurological diseases.
The Sun lab is interested in understanding the role of ion transporter proteins (Na+-K+ -Cl- cotransporter, Na+/H+ exchanger, and Na+/Ca2+ exchangers) in ionic dysregulation and neurodegeneration associated with stroke, traumatic brain injury, or glioma tumor. In particular, we study how changes of cytosolic ionic concentrations (Na+, H+, Ca2+) as well as organelle Ca2+ (ER and mitochondria) cause cell death and proinflammatory responses in ischemic or traumatic brain damage. We are also interested in studying Glioblastoma multiforme (GBM) brain tumor. We investigate how the Cl- cotransporter functions in regulation of intracellular Cl- and cell volume in GBM cancer cell survival and migration. These ion transport proteins may be potential ''targets'' for therapeutic interventions for these diseases. The Sun lab includes postdoc fellows, graduate students, and undergraduate students. The research employs in vitro cell culture models and in vivo animal models. A variety of biochemical assays, and live cell imaging techniques are used in our research.
Begum, G., Kintner, D., Liu, Y., Cramer, S.W. and Sun, Dandan. DHA inhibits ER Ca2+ release and ER stress in astrocytes following in vitro ischemia. J. Neurochem. 120: 622-630, 2012.
Algharabli, J., Kintner, D.B., Wang, Q., Begum, G., Clark, P.A., Yang, S., Lin, S., Kahle, K.T., Kuo, J.S. and Sun, Dandan. Inhibition of Na+-K+-2Cl¬- cotransporter isoform 1 accelerates temozolomide-mediated apoptosis in glioblastoma cancer cells. Cell Physiol and Biochem. 30: 33-48, 2012.
Liu, Y., Kintner, D.B., Begum, G., Algharabli, J., Cengiz, P., Shull, G.E., Liu, X. and Sun, Dandan. ER Ca2+ signaling and mitochondrial Cyt c release in astrocytes following oxygen and glucose deprivation. J Neurochem. 114: 1435-46, 2010.
Kintner, D.B., Chen, X., Chiu, S., Baba, A., Matsuda, T., Cohen, M., Orlowski, J., Taunton, J. and Sun, Dandan. Excessive Na+/H+ exchange in disruption of dendritic Na+ and Ca2+ homeostasis and mitochondrial dysfunction following in vitro ischemia. J Biol Chem. 285 (45): 35155-68, 2010.
Liu, Y., Kintner, D.B., Chanana, V., Algharabli, J., Chen, X., Gao, Y., Chen, J., Ferrazzano, P., Olson, J.K. and Sun, Dandan. Activation of microglia depends on Na+/H+ exchange-mediated H+ homeostasis. J. Neurosci. 30 (45): 15210-20, 2010.